{"product_id":"proteintech-19537-1-ap","title":"Proteintech, 19537-1-AP, CHKA-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CHKA-Specific (19537-1-AP) by Proteintech is a Polyclonal antibody targeting CHKA-Specific in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n19537-1-AP targets CHKA-Specific in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells,  COLO 320 cells,  human testis tissue,  mouse colon tissue,  mouse testis tissue,  Raji cells\nPositive IHC detected in: human breast cancer tissue,  human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCholine kinase alpha, also named as CHK, CKI and CK, belongs to the choline\/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: choline kinase alpha\nCalculated Molecular Weight: 52 kDa\nObserved Molecular Weight: 50 kDa\nGenBank Accession Number: NM_001277\nGene Symbol: CHKA\nGene ID (NCBI): 1119\nRRID: AB_10792247\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P35790\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46886520389801,"sku":"19537-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19537-1-ap","provider":"Iright","version":"1.0","type":"link"}