{"product_id":"proteintech-19650-1-ap","title":"Proteintech, 19650-1-AP, MUTYH Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MUTYH (19650-1-AP) by Proteintech is a Polyclonal antibody targeting MUTYH in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19650-1-AP targets MUTYH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  COLO 320 cells,  Jurkat cells,  K-562 cells,  mouse thymus tissue,  Raji cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: mouse liver tissue, human kidney tissue,  human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMUTYH(MutY homolog) is also named as MYH and belongs to the Nth\/MutY family. The gene enconde an A\/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7526 Product name: Recombinant human MUTYH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 187-535 aa of BC003178 Sequence: MPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ Predict reactive species\nFull Name: mutY homolog (E. coli)\nCalculated Molecular Weight: 60 kDa\nObserved Molecular Weight: 53-64 kDa\nGenBank Accession Number: BC003178\nGene Symbol: MUTYH\nGene ID (NCBI): 4595\nRRID: AB_10694143\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UIF7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869474476201,"sku":"19650-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19650-1-ap","provider":"Iright","version":"1.0","type":"link"}