{"product_id":"proteintech-19694-1-ap","title":"Proteintech, 19694-1-AP, INPP5D Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe INPP5D (19694-1-AP) by Proteintech is a Polyclonal antibody targeting INPP5D in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19694-1-AP targets INPP5D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Daudi cells, Ramos cells,  Raji cells,  THP-1 cells\nPositive IP detected in: Ramos cells\nPositive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\n\u003cb\u003eBackground Information\u003c\/b\u003e\nINPP5D, also named as SHIP, SHIP1, SIP-145 and hp51CN, belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. INPP5D is phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. INPP5D acts as a negative regulator of B-cell antigen receptor signaling. It mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune\/hematopoietic cell receptor systems. INPP5D acts as a negative regulator of myeloid cell proliferation\/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb\/beta-3 signaling in platelets and JNK signaling in B-cells. INPP5D regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It is involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. It is a key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. It modulates FCGR3\/CD16-mediated cytotoxicity in NK cells. It mediates the activin\/TGF-beta-induced apoptosis through its Smad-dependent expression. INPP5D may also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. This antibody is specific to INPP5D.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: inositol polyphosphate-5-phosphatase, 145kDa\nCalculated Molecular Weight: 133 kDa\nObserved Molecular Weight: 145 kDa\nGenBank Accession Number: NM_001017915\nGene Symbol: INPP5D\nGene ID (NCBI): 3635\nRRID: AB_10667407\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q92835\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868983120041,"sku":"19694-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19694-1-ap","provider":"Iright","version":"1.0","type":"link"}