{"product_id":"proteintech-19887-1-ap","title":"Proteintech, 19887-1-AP, NF90\/ILF3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NF90\/ILF3 (19887-1-AP) by Proteintech is a Polyclonal antibody targeting NF90\/ILF3 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n19887-1-AP targets NF90\/ILF3 in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Jurkat cells,  mouse spleen tissue,  K-562 cells,  Raji cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human cervical cancer tissue,  human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13707 Product name: Recombinant human NF90,ILF3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 8-343 aa of BC064836 Sequence: VNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKM Predict reactive species\nFull Name: interleukin enhancer binding factor 3, 90kDa\nCalculated Molecular Weight: 894 aa, 95 kDa\nObserved Molecular Weight: 90 kDa, 110 kDa\nGenBank Accession Number: BC064836\nGene Symbol: ILF3\nGene ID (NCBI): 3609\nRRID: AB_10666431\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q12906\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868855586985,"sku":"19887-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19887-1-ap","provider":"Iright","version":"1.0","type":"link"}