{"product_id":"proteintech-19930-1-ap","title":"Proteintech, 19930-1-AP, RBM5 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RBM5 (19930-1-AP) by Proteintech is a Polyclonal antibody targeting RBM5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19930-1-AP targets RBM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PC-3 cells\nPositive IHC detected in: human stomach tissue, human lung tissue,  rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5\/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2\/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13908 Product name: Recombinant human RBM5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC002957 Sequence: MGSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSDRSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSRGFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFEDWLCNKCCLNNFRKRLKCFRCGADKFDSEQEVPPGTTESVQSVDYYCDTIILRNIAPHTVVDSIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQLSSAMDASQLLQILQSLHPPLKIDGKTIGVDFAKSARKDLVLSDGNRVSAFSVASTAIAAAQWSSTQSQS Predict reactive species\nFull Name: RNA binding motif protein 5\nCalculated Molecular Weight: 815 aa, 92 kDa\nObserved Molecular Weight: 110-115 kDa\nGenBank Accession Number: BC002957\nGene Symbol: RBM5\nGene ID (NCBI): 10181\nRRID: AB_2878625\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P52756\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869426733225,"sku":"19930-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19930-1-ap","provider":"Iright","version":"1.0","type":"link"}