{"product_id":"proteintech-19943-1-ap","title":"Proteintech, 19943-1-AP, ERBB4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ERBB4 (19943-1-AP) by Proteintech is a Polyclonal antibody targeting ERBB4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n19943-1-AP targets ERBB4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, MCF-7 cells,  rat brain tissue\nPositive IHC detected in: mouse lung tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)\nCalculated Molecular Weight: 147 kDa\nObserved Molecular Weight: 180 kDa, 147 kDa\nGenBank Accession Number: NM_001042599\nGene Symbol: ERBB4\nGene ID (NCBI): 2066\nRRID: AB_10646486\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15303\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868876361897,"sku":"19943-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-19943-1-ap","provider":"Iright","version":"1.0","type":"link"}