{"product_id":"proteintech-20145-1-ap","title":"Proteintech, 20145-1-AP, SHP2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SHP2 (20145-1-AP) by Proteintech is a Polyclonal antibody targeting SHP2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n20145-1-AP targets SHP2 in WB, IHC, IF\/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, A431 cells,  HeLa cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human breast cancer tissue, human gliomas tissue,  human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13660 Product name: Recombinant human PTPN11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC008692 Sequence: MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTT Predict reactive species\nFull Name: protein tyrosine phosphatase, non-receptor type 11\nCalculated Molecular Weight: 597 aa, 68 kDa\nObserved Molecular Weight: 68-75 kDa\nGenBank Accession Number: BC008692\nGene Symbol: PTPN11\nGene ID (NCBI): 5781\nRRID: AB_10699877\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q06124\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868849885353,"sku":"20145-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-20145-1-ap","provider":"Iright","version":"1.0","type":"link"}