{"product_id":"proteintech-20702-1-ap","title":"Proteintech, 20702-1-AP, IRS2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe IRS2 (20702-1-AP) by Proteintech is a Polyclonal antibody targeting IRS2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n20702-1-AP targets IRS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, rat brain tissue\nPositive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: IRS2\nCalculated Molecular Weight: 137 kDa\nObserved Molecular Weight: 150-185 kDa\nGenBank Accession Number: NM_003749\nGene Symbol: IRS2\nGene ID (NCBI): 8660\nRRID: AB_2918067\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y4H2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868918698153,"sku":"20702-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-20702-1-ap","provider":"Iright","version":"1.0","type":"link"}