{"product_id":"proteintech-20960-1-ap","title":"Proteintech, 20960-1-AP, HIF-1 alpha Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HIF-1 alpha (20960-1-AP) by Proteintech is a Polyclonal antibody targeting HIF-1 alpha in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n20960-1-AP targets HIF-1 alpha in WB, IHC, IF\/ICC, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Cobalt Chloride treated HeLa cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human thyroid cancer tissue, human kidney tissue,  human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, chicken, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15198 Product name: Recombinant human HIF1a protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 574-799 aa of BC012527 Sequence: LRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYD Predict reactive species\nFull Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)\nCalculated Molecular Weight: 826 aa, 93 kDa\nObserved Molecular Weight: 120 kDa\nGenBank Accession Number: BC012527\nGene Symbol: HIF1A\nGene ID (NCBI): 3091\nRRID: AB_10732601\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q16665\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868816363689,"sku":"20960-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-20960-1-ap","provider":"Iright","version":"1.0","type":"link"}