{"product_id":"proteintech-21093-1-ap","title":"Proteintech, 21093-1-AP, AFAP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AFAP1 (21093-1-AP) by Proteintech is a Polyclonal antibody targeting AFAP1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n21093-1-AP targets AFAP1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: DU 145 cells, mouse brain tissue,  rat brain tissue,  HeLa cells,  MCF-7 cells,  PC-3 cells\nPositive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nActin filament-associated protein 1 (AFAP1) encodes a motor fiber-associated protein, which organizes a network for linking other proteins, including SRC proto-oncogene, non-receptor tyrosine kinase (Src) and protein kinase C . A long non-coding RNA, namely AFAP1-antisense RNA 1 (AS1), is transcribed from the antisense strand of this gene and potentially regulates its expression.(PMID: 31086663, PMID: 35193469)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15346 Product name: Recombinant human AFAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 382-730 aa of BC032777 Sequence: DLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT Predict reactive species\nFull Name: actin filament associated protein 1\nCalculated Molecular Weight: 730 aa, 81 kDa\nObserved Molecular Weight: 110 kDa\nGenBank Accession Number: BC032777\nGene Symbol: AFAP1\nGene ID (NCBI): 60312\nRRID: AB_10732810\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8N556\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868962541737,"sku":"21093-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-21093-1-ap","provider":"Iright","version":"1.0","type":"link"}