{"product_id":"proteintech-21097-1-ap","title":"Proteintech, 21097-1-AP, PRAS40 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PRAS40 (21097-1-AP) by Proteintech is a Polyclonal antibody targeting PRAS40 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n21097-1-AP targets PRAS40 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells,  HeLa cells\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K\/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis,  as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB\/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine\/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15355 Product name: Recombinant human PRAS40 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC051844 Sequence: MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY Predict reactive species\nFull Name: AKT1 substrate 1 (proline-rich)\nCalculated Molecular Weight: 27 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC051844\nGene Symbol: PRAS40\nGene ID (NCBI): 84335\nRRID: AB_2878811\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96B36\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869424505001,"sku":"21097-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-21097-1-ap","provider":"Iright","version":"1.0","type":"link"}