{"product_id":"proteintech-21175-1-ap","title":"Proteintech, 21175-1-AP, LRPPRC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LRPPRC (21175-1-AP) by Proteintech is a Polyclonal antibody targeting LRPPRC in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n21175-1-AP targets LRPPRC in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, mouse heart tissue,  HepG2 cells,  mouse skeletal muscle tissue,  mouse kidney tissue,  mouse liver tissue,  HeLa cells,  rat kidney tissue,  rat liver tissue,  rat heart tissue,  PC-12 cells,  SH-SY5Y cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human kidney tissue, human colon tissue,  human heart tissue,  mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells, HepG2 cells,  HeLa cells,  HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15452 Product name: Recombinant human LRPPRC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1014-1394 aa of BC050311 Sequence: LWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS Predict reactive species\nFull Name: leucine-rich PPR-motif containing\nCalculated Molecular Weight: 1394 aa, 158 kDa\nObserved Molecular Weight: 130 kDa\nGenBank Accession Number: BC050311\nGene Symbol: LRPPRC\nGene ID (NCBI): 10128\nRRID: AB_10733879\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P42704\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868847394985,"sku":"21175-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-21175-1-ap","provider":"Iright","version":"1.0","type":"link"}