{"product_id":"proteintech-21207-1-ap","title":"Proteintech, 21207-1-AP, TET2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TET2 (21207-1-AP) by Proteintech is a Polyclonal antibody targeting TET2 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n21207-1-AP targets TET2 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HL-60 cells,  HEK-293 cells,  HeLa cells,  HepG2 cells,  K-562 cells\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells, RAW 264.7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, goat, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag15583 Product name: Recombinant human TET2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC150180 Sequence: MEQDRTNHVEGNRLSPFLIPSPPICQTERLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGE Predict reactive species\nFull Name: tet oncogene family member 2\nCalculated Molecular Weight: 2002 aa, 224 kDa\nObserved Molecular Weight: 130 kDa, 250 kDa\nGenBank Accession Number: BC150180\nGene Symbol: TET2\nGene ID (NCBI): 54790\nRRID: AB_10734584\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q6N021\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868833075369,"sku":"21207-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-21207-1-ap","provider":"Iright","version":"1.0","type":"link"}