{"product_id":"proteintech-21854-1-ap","title":"Proteintech, 21854-1-AP, NR3C2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NR3C2 (21854-1-AP) by Proteintech is a Polyclonal antibody targeting NR3C2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n21854-1-AP targets NR3C2 in WB, IHC, IF\/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse kidney tissue, HEK-293 cells,  HeLa cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human kidney tissue, human colon tissue,  mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid\/thyroid \/retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag16410 Product name: Recombinant human NR3C2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC111758 Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAIVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA Predict reactive species\nFull Name: nuclear receptor subfamily 3, group C, member 2\nCalculated Molecular Weight: 984 aa, 107 kDa\nObserved Molecular Weight: 94-110 kDa\nGenBank Accession Number: BC111758\nGene Symbol: NR3C2\nGene ID (NCBI): 4306\nRRID: AB_10858785\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P08235\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868856930473,"sku":"21854-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-21854-1-ap","provider":"Iright","version":"1.0","type":"link"}