{"product_id":"proteintech-22246-1-ap","title":"Proteintech, 22246-1-AP, TBP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TBP (22246-1-AP) by Proteintech is a Polyclonal antibody targeting TBP in WB, IF\/ICC, ELISA applications with reactivity to human,  mouse samples\n22246-1-AP targets TBP in WB, IF\/ICC, ELISA applications and shows reactivity with human,  mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells,  COLO 320 cells,  HeLa cells,  mouse colon tissue,  SKOV-3 cells\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17740 Product name: Recombinant human TBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC110341 Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPS Predict reactive species\nFull Name: TATA box binding protein\nCalculated Molecular Weight: 338 aa, 38 kDa\nObserved Molecular Weight: mouse\/rat 33-36 kDa and human 37-43kDa\nGenBank Accession Number: BC110341\nGene Symbol: TBP\nGene ID (NCBI): 6908\nRRID: AB_2879046\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P20226\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869611708585,"sku":"22246-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-22246-1-ap","provider":"Iright","version":"1.0","type":"link"}