{"product_id":"proteintech-22261-1-ap","title":"Proteintech, 22261-1-AP, ESPL1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ESPL1 (22261-1-AP) by Proteintech is a Polyclonal antibody targeting ESPL1 in WB, ELISA applications with reactivity to human samples\n22261-1-AP targets ESPL1 in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, Jurkat cells,  Raji cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: extra spindle pole bodies homolog 1 (S. cerevisiae)\nCalculated Molecular Weight: 233 kDa\nObserved Molecular Weight: 170 kDa\nGenBank Accession Number: NM_012291\nGene Symbol: ESPL1\nGene ID (NCBI): 9700\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14674\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887628046505,"sku":"22261-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-22261-1-ap","provider":"Iright","version":"1.0","type":"link"}