{"product_id":"proteintech-23191-1-ap","title":"Proteintech, 23191-1-AP, MMADHC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MMADHC (23191-1-AP) by Proteintech is a Polyclonal antibody targeting MMADHC in WB, IHC, ELISA applications with reactivity to human,  mouse samples\n23191-1-AP targets MMADHC in WB, IHC, ELISA applications and shows reactivity with human,  mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  MCF-7 cells,  NIH3T3 cells\nPositive IHC detected in: human skeletal muscle tissue,  human heart tissue,  human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag19343 Product name: Recombinant human MMADHC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-291 aa of BC022859 Sequence: ESHVAAAPPDICSRTVWPDETMGPFGPQDQRFQLPGNIGFDCHLNGTASQKKSLVHKTLPDVLAEPLSSERHEFVMAQYVNEFQGNDAPVEQEINSAETYFESARVECAIQTCPELLRKDFESLFPEVANGKLMILTVTQKTKNDMTVWSEEVEIEREVLLEKFINGAKEICYALRAEGYWADFIDPSSGLAFFGPYTNNTLFETDERYRHLGFSVDDLGCCKVIRHSLWGTHVVVGSIFTNATPDSHIMK Predict reactive species\nFull Name: methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria\nCalculated Molecular Weight: 296 aa, 33 kDa\nObserved Molecular Weight: 33 kDa\nGenBank Accession Number: BC022859\nGene Symbol: MMADHC\nGene ID (NCBI): 27249\nRRID: AB_2879229\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H3L0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887721042089,"sku":"23191-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-23191-1-ap","provider":"Iright","version":"1.0","type":"link"}