{"product_id":"proteintech-23599-1-ap","title":"Proteintech, 23599-1-AP, RPS25 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RPS25 (23599-1-AP) by Proteintech is a Polyclonal antibody targeting RPS25 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n23599-1-AP targets RPS25 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HeLa cells,  K-562 cells,  MCF-7 cells\nPositive IHC detected in: human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRibosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7890 Product name: Recombinant human RPS25 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-125 aa of BC004986 Sequence: MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA Predict reactive species\nFull Name: ribosomal protein S25\nCalculated Molecular Weight: 14 kDa\nObserved Molecular Weight: 15-17 kDa\nGenBank Accession Number: BC004986\nGene Symbol: RPS25\nGene ID (NCBI): 6230\nRRID: AB_11183757\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P62851\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869590147241,"sku":"23599-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-23599-1-ap","provider":"Iright","version":"1.0","type":"link"}