{"product_id":"proteintech-24559-1-ap","title":"Proteintech, 24559-1-AP, USP35 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe USP35 (24559-1-AP) by Proteintech is a Polyclonal antibody targeting USP35 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n24559-1-AP targets USP35 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HeLa cells,  RAW 264.7 cells\nPositive IHC detected in: human colon cancer tissue, mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUSP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC\/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag20002 Product name: Recombinant human USP35 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-342 aa of BC131489 Sequence: MPGLRRRASWRVSIPGSWPSQTRARLVSSTWATHAMSTASFRPYSWRLSSFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSE Predict reactive species\nFull Name: ubiquitin specific peptidase 35\nCalculated Molecular Weight: 1018 aa, 113 kDa\nObserved Molecular Weight: 84 kDa\nGenBank Accession Number: BC131489\nGene Symbol: USP35\nGene ID (NCBI): 57558\nRRID: AB_2879608\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9P2H5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868975386793,"sku":"24559-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-24559-1-ap","provider":"Iright","version":"1.0","type":"link"}