{"product_id":"proteintech-25199-1-ap","title":"Proteintech, 25199-1-AP, AKAP12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AKAP12 (25199-1-AP) by Proteintech is a Polyclonal antibody targeting AKAP12 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n25199-1-AP targets AKAP12 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat brain tissue\nPositive IHC detected in: mouse brain tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag17769 Product name: Recombinant human AKAP12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1335-1684 aa of BC157067 Sequence: ETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES Predict reactive species\nFull Name: A kinase (PRKA) anchor protein 12\nCalculated Molecular Weight: 1782 aa, 191 kDa\nObserved Molecular Weight: 200-300 kDa\nGenBank Accession Number: BC157067\nGene Symbol: AKAP12\nGene ID (NCBI): 9590\nRRID: AB_2879954\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q02952\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868915486889,"sku":"25199-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-25199-1-ap","provider":"Iright","version":"1.0","type":"link"}