{"product_id":"proteintech-25896-1-ap","title":"Proteintech, 25896-1-AP, MTR Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MTR (25896-1-AP) by Proteintech is a Polyclonal antibody targeting MTR in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n25896-1-AP targets MTR in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A375 cells, mouse pancreas tissue,  HEK-293 cells,  HepG2 cells,  L02 cells\nPositive IHC detected in: human cervical cancer tissue, human heart tissue,  human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A375 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMTR (Methionine synthase), one of two B12-dependent mammalian enzymes, catalyzes a methyl transfer from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate (PMID: 9587027, 9013615). Methionine synthase levels in mammalian cells are influenced by folate status, and the ability of cells to grow in the absence of methionine is partly rate-limited by synthase activity levels. MTR loss depletes cells and tumors of reduced folate species (PMID: 34799699)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag23156 Product name: Recombinant human MTR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 917-1265 aa of BC130616 Sequence: RQGHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD Predict reactive species\nFull Name: 5-methyltetrahydrofolate-homocysteine methyltransferase\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC130616\nGene Symbol: MTR\nGene ID (NCBI): 4548\nRRID: AB_2880287\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q99707\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868898250921,"sku":"25896-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-25896-1-ap","provider":"Iright","version":"1.0","type":"link"}