{"product_id":"proteintech-27320-1-ap","title":"Proteintech, 27320-1-AP, APP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe APP (27320-1-AP) by Proteintech is a Polyclonal antibody targeting APP in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n27320-1-AP targets APP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: fetal human brain tissue, HEK-293T cells,  U-251 cells,  mouse brain tissue,  HeLa cells,  rat brain tissue,  MDA-MB-231 cells\nPositive IHC detected in: mouse brain tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26299 Product name: Recombinant human APP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 175-289 aa of BC004369 Sequence: GIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRE Predict reactive species\nFull Name: amyloid beta (A4) precursor protein\nCalculated Molecular Weight: 87 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC004369\nGene Symbol: APP\nGene ID (NCBI): 351\nRRID: AB_2880842\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05067\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869393014953,"sku":"27320-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-27320-1-ap","provider":"Iright","version":"1.0","type":"link"}