{"product_id":"proteintech-27917-1-ap","title":"Proteintech, 27917-1-AP, CRM1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CRM1 (27917-1-AP) by Proteintech is a Polyclonal antibody targeting CRM1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n27917-1-AP targets CRM1 in WB, IHC, IF\/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, C6 cells,  HeLa cells,  MCF-7 cells,  NIH\/3T3 cells,  K-562 cells,  HT-1080 cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag27207 Product name: Recombinant human XPO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 301-540 aa of BC032847 Sequence: NTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAII Predict reactive species\nFull Name: exportin 1 (CRM1 homolog, yeast)\nCalculated Molecular Weight: 1071 aa, 123 kDa\nObserved Molecular Weight: 123 kDa\nGenBank Accession Number: BC032847\nGene Symbol: CRM1\nGene ID (NCBI): 7514\nRRID: AB_2881009\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14980\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868951597225,"sku":"27917-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-27917-1-ap","provider":"Iright","version":"1.0","type":"link"}