{"product_id":"proteintech-28055-1-ap","title":"Proteintech, 28055-1-AP, NKCC1\/SLC12A2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NKCC1\/SLC12A2 (28055-1-AP) by Proteintech is a Polyclonal antibody targeting NKCC1\/SLC12A2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n28055-1-AP targets NKCC1\/SLC12A2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: unboiled HeLa cells\nPositive IHC detected in: human breast cancer tissue, human colon cancer tissue,  human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947).  The observed molecular weight of  NKCC1 is about 140 kDa,  glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag27895 Product name: Recombinant human SLC12A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC033003 Sequence: MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESK Predict reactive species\nFull Name: solute carrier family 12 (sodium\/potassium\/chloride transporters), member 2\nCalculated Molecular Weight: 131 kDa\nObserved Molecular Weight: 150-160 kDa\nGenBank Accession Number: BC033003\nGene Symbol: NKCC1\/SLC12A2\nGene ID (NCBI): 6558\nRRID: AB_2881048\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P55011\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887737884841,"sku":"28055-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-28055-1-ap","provider":"Iright","version":"1.0","type":"link"}