{"product_id":"proteintech-28509-1-ap","title":"Proteintech, 28509-1-AP, NOC2L Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NOC2L (28509-1-AP) by Proteintech is a Polyclonal antibody targeting NOC2L in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human, mouse samples\n28509-1-AP targets NOC2L in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U2OS cells, HEK-293T cells,  HuH-7 cells,  mouse cerebellum tissue,  mouse stomach tissue\nPositive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300\/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag26952 Product name: Recombinant human NOC2L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC003555 Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA Predict reactive species\nFull Name: nucleolar complex associated 2 homolog (S. cerevisiae)\nCalculated Molecular Weight: 749 aa, 85 kDa\nObserved Molecular Weight: 85 kDa\nGenBank Accession Number: BC003555\nGene Symbol: NOC2L\nGene ID (NCBI): 26155\nRRID: AB_2881160\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y3T9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887739556009,"sku":"28509-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-28509-1-ap","provider":"Iright","version":"1.0","type":"link"}