{"product_id":"proteintech-28554-1-ap","title":"Proteintech, 28554-1-AP, PICALM Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PICALM (28554-1-AP) by Proteintech is a Polyclonal antibody targeting PICALM in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n28554-1-AP targets PICALM in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HeLa cells,  K-562 cells,  NIH\/3T3 cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag29505 Product name: Recombinant human PICALM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC064357 Sequence: MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTT Predict reactive species\nFull Name: phosphatidylinositol binding clathrin assembly protein\nCalculated Molecular Weight: 71 kDa\nObserved Molecular Weight: 60-70 kDa\nGenBank Accession Number: BC064357\nGene Symbol: PICALM\nGene ID (NCBI): 8301\nRRID: AB_2918175\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13492\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887762329769,"sku":"28554-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-28554-1-ap","provider":"Iright","version":"1.0","type":"link"}