{"product_id":"proteintech-28939-1-ap","title":"Proteintech, 28939-1-AP, Phospho-eNOS (Thr495) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-eNOS (Thr495) (28939-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-eNOS (Thr495) in WB, ELISA applications with reactivity to human samples\n28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: VEGF treated HUVEC cells, EGF treated HUVEC cells,  Cobalt Chloride treated HUVEC cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEndothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: nitric oxide synthase 3 (endothelial cell)\nCalculated Molecular Weight: 133 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC063294\nGene Symbol: eNOS\nGene ID (NCBI): 4846\nRRID: AB_2881234\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P29474\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868943896745,"sku":"28939-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-28939-1-ap","provider":"Iright","version":"1.0","type":"link"}