{"product_id":"proteintech-29546-1-ap","title":"Proteintech, 29546-1-AP, Phospho-PERK\/EIF2AK3 (Ser719) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-PERK\/EIF2AK3 (Ser719) (29546-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PERK\/EIF2AK3 (Ser719) in WB, ELISA applications with reactivity to human samples\n29546-1-AP targets Phospho-PERK\/EIF2AK3 (Ser719) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: λ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, zebrafish, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: eukaryotic translation initiation factor 2-alpha kinase 3\nCalculated Molecular Weight: 1116 aa, 125 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC126354\nGene Symbol: PERK\nGene ID (NCBI): 9451\nRRID: AB_2935416\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NZJ5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868855750825,"sku":"29546-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-29546-1-ap","provider":"Iright","version":"1.0","type":"link"}