{"product_id":"proteintech-29562-1-ap","title":"Proteintech, 29562-1-AP, Phospho-PKC Delta (Ser359) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-PKC Delta (Ser359) (29562-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PKC Delta (Ser359) in WB, IF\/ICC, ELISA applications with reactivity to Human samples\n29562-1-AP targets Phospho-PKC Delta (Ser359) in WB, IF\/ICC, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: λ phosphatase treated Jurkat cells\nPositive IF\/ICC detected in: λ phosphatase treated A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nProtein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine\/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ\/ι), which respond to neither DAG nor calcium. PKCδ, unlike other members of the PKC family, is unique in its regulation by tyrosine phosphorylation on multiple sites that determine activation, localization, and substrate specificity. PKCδ is activated by inflammatory mediators involved in the inflammatory response including lipopolysaccharide (LPS), tumor necrosis factor (TNF) and interleukin-1 (IL-1). PKCδ activation requires multi-phosphorylation steps which triggers translocation from the cell cytosol to different subcellular compartments. (PMID: 30095599, PMID: 31323909, PMID: 30917487)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: protein kinase C, delta\nCalculated Molecular Weight: 78 kDa\nObserved Molecular Weight: 70 kDa\nGenBank Accession Number: BC043350\nGene Symbol: PKC Delta\nGene ID (NCBI): 5580\nRRID: AB_2923597\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q05655\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869571731625,"sku":"29562-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-29562-1-ap","provider":"Iright","version":"1.0","type":"link"}