{"product_id":"proteintech-29583-1-ap","title":"Proteintech, 29583-1-AP, Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-PDH E1 Alpha (Ser300) (29583-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PDH E1 Alpha (Ser300) in WB, IF\/ICC, ELISA applications with reactivity to Human samples\n29583-1-AP targets Phospho-PDH E1 Alpha (Ser300) in WB, IF\/ICC, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells\nPositive IF\/ICC detected in: λ phosphatase treated HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:14000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis.  (PMID: 30993888, PMID: 34749809)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: pyruvate dehydrogenase (lipoamide) alpha 1\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC002406\nGene Symbol: PDHA1\nGene ID (NCBI): 5160\nRRID: AB_2935419\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P08559\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869422801065,"sku":"29583-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-29583-1-ap","provider":"Iright","version":"1.0","type":"link"}