{"product_id":"proteintech-29895-1-ap","title":"Proteintech, 29895-1-AP, HMGA1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HMGA1 (29895-1-AP) by Proteintech is a Polyclonal antibody targeting HMGA1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n29895-1-AP targets HMGA1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, MKN-45 cells\nPositive IHC detected in: human prostate cancer tissue, human pancreas cancer tissue,  human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HUVEC cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag31866 Product name: Recombinant human HMGA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-77 aa of BC071864 Sequence: SESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTT Predict reactive species\nFull Name: high mobility group AT-hook 1\nObserved Molecular Weight: 20 kDa\nGenBank Accession Number: BC071864\nGene Symbol: HMGA1\nGene ID (NCBI): 3159\nRRID: AB_2923618\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P17096\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868967555241,"sku":"29895-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-29895-1-ap","provider":"Iright","version":"1.0","type":"link"}