{"product_id":"proteintech-29993-1-ap","title":"Proteintech, 29993-1-AP, GAA Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GAA (29993-1-AP) by Proteintech is a Polyclonal antibody targeting GAA in WB, IHC, ELISA applications with reactivity to Human, Mouse samples\n29993-1-AP targets GAA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: DU 145 cells, LNCaP cells,  PC-13 cells\nPositive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag28970 Product name: Recombinant human GAA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 38-230 aa of BC040431 Sequence: VPRELSGSSPVLEETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQEQCEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPLYSVEFSEEPFGVIVRRQLDGRV Predict reactive species\nFull Name: glucosidase, alpha; acid\nCalculated Molecular Weight: 105 kDa\nObserved Molecular Weight: 110 kDa\nGenBank Accession Number: BC040431\nGene Symbol: GAA\nGene ID (NCBI): 2548\nRRID: AB_2923627\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P10253\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887645184169,"sku":"29993-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-29993-1-ap","provider":"Iright","version":"1.0","type":"link"}