{"product_id":"proteintech-30332-1-ap","title":"Proteintech, 30332-1-AP, ARL13B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ARL13B (30332-1-AP) by Proteintech is a Polyclonal antibody targeting ARL13B in WB, IF\/ICC, IP, ELISA applications with reactivity to human samples\n30332-1-AP targets ARL13B in WB, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HepG2 cells,  K-562 cells,  L02 cells\nPositive IP detected in: L02 cells\nPositive IF\/ICC detected in: MDCK cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and  the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).  The second antibody is used against guinea pigs.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Guinea Pig \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species\nFull Name: ADP-ribosylation factor-like 13B\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 48-55 kDa\nGenBank Accession Number: BC094725\nGene Symbol: ARL13B\nGene ID (NCBI): 200894\nRRID: AB_3086296\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q3SXY8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46885039243433,"sku":"30332-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-30332-1-ap","provider":"Iright","version":"1.0","type":"link"}