{"product_id":"proteintech-30373-1-ap","title":"Proteintech, 30373-1-AP, RB1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RB1 (30373-1-AP) by Proteintech is a Polyclonal antibody targeting RB1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n30373-1-AP targets RB1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells, HT-1080 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor.  RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: retinoblastoma 1\nCalculated Molecular Weight: 928 aa, 106 kDa\nObserved Molecular Weight: 110 kDa\nGenBank Accession Number: BC039060\nGene Symbol: RB1\nGene ID (NCBI): 5925\nRRID: AB_3086301\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P06400\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868843856041,"sku":"30373-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-30373-1-ap","provider":"Iright","version":"1.0","type":"link"}