{"product_id":"proteintech-31684-1-ap","title":"Proteintech, 31684-1-AP, ARFGEF1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ARFGEF1 (31684-1-AP) by Proteintech is a Polyclonal antibody targeting ARFGEF1 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n31684-1-AP targets ARFGEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: BxPC-3 cells, HEK-293T cells,  HeLa cells,  NIH\/3T3 cells,  mouse lung tissue\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nARFGEF1 (ADP ribosylation factor guanine nucleotide\nexchange factor 1), also known as BIG1 (Brefeldin A-inhibited\nguanine nucleotide exchange protein 1),  is one of the ADP\nribosylation factor (ARF) guanine nucleotide exchange\nfactor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag35629 Product name: Recombinant human ARFGEF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 212-420 aa of NM_001413184 Sequence: QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ Predict reactive species\nFull Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)\nCalculated Molecular Weight: 208kDa\nObserved Molecular Weight: 210 kDa\nGenBank Accession Number: NM_001413184\nGene Symbol: ARFGEF1\nGene ID (NCBI): 10565\nRRID: AB_3670078\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q9Y6D6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46885015093417,"sku":"31684-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-31684-1-ap","provider":"Iright","version":"1.0","type":"link"}