{"product_id":"proteintech-33563-1-ap","title":"Proteintech, 33563-1-AP, DZIP3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DZIP3 (33563-1-AP) by Proteintech is a Polyclonal antibody targeting DZIP3 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n33563-1-AP targets DZIP3 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: AC16 cells\nPositive IP detected in: HEK-293T cells\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDZIP3\/hRUL138 is a poorly characterized RNA-binding RING E3-ubiquitin ligase with functions in embryonic development. DZIP3 localizes in both the nucleus and cytoplasm (PMID: 33067265). \nDZIP3 was mainly reported to be an RNA-binding ligase that causes histone H2A monoubiquitination, which represses transcription. DZIP3 also regulates the differentiation of stem cells (PMID: 32796072).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag35815 Product name: Recombinant human DZIP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 631-862 aa of BC063882 Sequence: AEINKDGTSIPSESSTESLKDLQEVKSKQRKKKKTKNKKNKDSKEDQVPYVVEKEEQLRKEQANPHSVSRLIKDDASDVQEDSAMEDKFYSLDELHILDMIEQGSAGKVTTDYGETEKERLARQRQLYKLHYQCEDFKRQLRTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQIHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTMKTYVSKLNAETSRALTA Predict reactive species\nFull Name: DAZ interacting protein 3, zinc finger\nCalculated Molecular Weight: 1208 aa, 139 kDa\nObserved Molecular Weight: 139-150 kDa\nGenBank Accession Number: BC063882\nGene Symbol: DZIP3\nGene ID (NCBI): 9666\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity Purification\nUNIPROT ID: Q86Y13\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887398998185,"sku":"33563-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-33563-1-ap","provider":"Iright","version":"1.0","type":"link"}