{"product_id":"proteintech-51105-1-ap","title":"Proteintech, 51105-1-AP, HER2\/ErbB2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HER2\/ErbB2 (51105-1-AP) by Proteintech is a Polyclonal antibody targeting HER2\/ErbB2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n51105-1-AP targets HER2\/ErbB2 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  MCF-7 cells\nPositive IP detected in: SK-BR-3 cells\nPositive IHC detected in: human breast cancer tissue, Breast Cancer slidesNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and\/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro\/glioblastoma derived oncogene homolog (avian)\nCalculated Molecular Weight: 185 kDa\nObserved Molecular Weight: 185 kDa\nGenBank Accession Number: BC167147\nGene Symbol: HER2\/ErbB2\nGene ID (NCBI): 2064\nRRID: AB_2099262\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P04626\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869692383401,"sku":"51105-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-51105-1-ap","provider":"Iright","version":"1.0","type":"link"}