{"product_id":"proteintech-51151-1-ap","title":"Proteintech, 51151-1-AP, JNK Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe JNK (51151-1-AP) by Proteintech is a Polyclonal antibody targeting JNK in WB, IHC, IF\/ICC, ELISA applications with reactivity to human,  mouse samples\n51151-1-AP targets JNK in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human,  mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 4T1 cells, mouse brain tissue,  A431 cells,  HEK-293 cells,  HepG2 cells,  Jurkat cells,  Neuro-2a cells,  C6 cells,  NIH\/3T3 cells\nPositive IHC detected in: human colon cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFunctionJNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine\/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.Tissue specificityJNK1 is ubiquitously expressed.Involvement in diseaseJNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.IsoformsThere are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.Post-translational modificationsJNK1 is phosphorylated by MAPK kinases (MAPKK).Cellular localizationJNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, pig, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 8\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 44-48 kDa, 50-55 kDa\nGenBank Accession Number: NM_138982\nGene Symbol: JNK\nGene ID (NCBI): 5599\nRRID: AB_2140710\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P45983\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868826783913,"sku":"51151-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-51151-1-ap","provider":"Iright","version":"1.0","type":"link"}