{"product_id":"proteintech-55154-1-ap","title":"Proteintech, 55154-1-AP, LNPEP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LNPEP (55154-1-AP) by Proteintech is a Polyclonal antibody targeting LNPEP in WB, IHC, ELISA applications with reactivity to human samples\n55154-1-AP targets LNPEP in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human brain tissue,  COLO 320 cells,  human heart tissue\nPositive IHC detected in: human heart tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: leucyl\/cystinyl aminopeptidase\nCalculated Molecular Weight: 117 kDa\nObserved Molecular Weight: 117-125 kDa\nGenBank Accession Number: NM_005575\nGene Symbol: LNPEP\nGene ID (NCBI): 4012\nRRID: AB_10860904\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UIQ6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887705673897,"sku":"55154-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-55154-1-ap","provider":"Iright","version":"1.0","type":"link"}