{"product_id":"proteintech-55234-1-ap","title":"Proteintech, 55234-1-AP, ENO3-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ENO3-specific (55234-1-AP) by Proteintech is a Polyclonal antibody targeting ENO3-specific in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n55234-1-AP targets ENO3-specific in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, SH-SY5Y cells,  HepG2 cells,  mouse brain tissue,  Jurkat cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: mouse skeletal muscle tissue, human heart tissue,  human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: enolase 3 (beta, muscle)\nCalculated Molecular Weight: 47 kDa\nObserved Molecular Weight: 42-47 kDa\nGenBank Accession Number: NM_001976\nGene Symbol: ENO3\nGene ID (NCBI): 2027\nRRID: AB_10837363\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P13929\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868965621929,"sku":"55234-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-55234-1-ap","provider":"Iright","version":"1.0","type":"link"}