{"product_id":"proteintech-55238-1-ap","title":"Proteintech, 55238-1-AP, Caspase 12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Caspase 12 (55238-1-AP) by Proteintech is a Polyclonal antibody targeting Caspase 12 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n55238-1-AP targets Caspase 12 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  multi-cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human prostate hyperplasia tissue,  human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCaspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, hamster\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: caspase 12 (gene\/pseudogene)\nCalculated Molecular Weight: 39 kDa\nObserved Molecular Weight: 36-42 kDa, 50 kDa\nGenBank Accession Number: NR_000035\nGene Symbol: Caspase 12\nGene ID (NCBI): 120329\nRRID: AB_10892670\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868828651689,"sku":"55238-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-55238-1-ap","provider":"Iright","version":"1.0","type":"link"}