{"product_id":"proteintech-55374-1-ap","title":"Proteintech, 55374-1-AP, TRIM33 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TRIM33 (55374-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM33 in WB, IHC, ELISA applications with reactivity to human samples\n55374-1-AP targets TRIM33 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, MCF-7 cells,  PC-3 cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM\/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta\/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem\/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. \nThe calculated molecular weight of TRIM33 is 122kDa, but the  molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tripartite motif-containing 33\nCalculated Molecular Weight: 122 kDa\nObserved Molecular Weight: 140-150 kDa\nGenBank Accession Number: NM_015906\nGene Symbol: TRIM33\nGene ID (NCBI): 51592\nRRID: AB_11183035\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UPN9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868990754985,"sku":"55374-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-55374-1-ap","provider":"Iright","version":"1.0","type":"link"}