{"product_id":"proteintech-60075-1-ig","title":"Proteintech, 60075-1-Ig, Septin 2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Septin 2 (60075-1-Ig) by Proteintech is a Monoclonal antibody targeting Septin 2 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n60075-1-Ig targets Septin 2 in WB, IHC, IF\/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, Jurkat cells,  fetal human brain tissue,  human testis tissue,  HeLa cells,  HEK-293 cells,  HepG2 cells,  K-562 cells,  HSC-T6 cells,  NIH\/3T3 cells,  4T1 cells,  MCF-7 cells,  HSCT6 cells,  ROS1728 cells,  Neuro-2A cells\nPositive IHC detected in: human renal cell carcinoma tissue,  human brain tissue,  human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: K-562 cells, hTERT-RPE cells\nPositive FC (Intra) detected in: K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:50-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSeptin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, canine\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag1946 Product name: Recombinant human SEPT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC014455 Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV Predict reactive species\nFull Name: septin 2\nCalculated Molecular Weight: 41 kDa\nObserved Molecular Weight: 42-45 kDa\nGenBank Accession Number: BC014455\nGene Symbol: Septin 2\nGene ID (NCBI): 4735\nRRID: AB_2187016\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q15019\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887988691113,"sku":"60075-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-60075-1-ig","provider":"Iright","version":"1.0","type":"link"}