{"product_id":"proteintech-60131-1-ig","title":"Proteintech, 60131-1-Ig, Neudesin\/NENF Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Neudesin\/NENF (60131-1-Ig) by Proteintech is a Monoclonal antibody targeting Neudesin\/NENF in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, pig samples\n60131-1-Ig targets Neudesin\/NENF in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, human brain tissue,  HepG2 cells,  LNCaP cells,  A549 cells,  HCT 116 cells,  Jurkat cells,  pig brain tissue\nPositive IHC detected in: human cerebellum tissue, human colon tissue,  human colon cancer tissue,  human heart tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:20-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNeudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine\/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag8387 Product name: Recombinant human NENF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 33-172 aa of BC008823 Sequence: QTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF Predict reactive species\nFull Name: neuron derived neurotrophic factor\nCalculated Molecular Weight: 172 aa, 19 kDa\nObserved Molecular Weight: 19 kDa, 16 kDa\nGenBank Accession Number: BC008823\nGene Symbol: NENF\nGene ID (NCBI): 29937\nRRID: AB_2150987\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9UMX5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887977189545,"sku":"60131-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-60131-1-ig","provider":"Iright","version":"1.0","type":"link"}