{"product_id":"proteintech-60203-2-ig","title":"Proteintech, 60203-2-Ig, AKT Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe AKT (60203-2-Ig) by Proteintech is a Monoclonal antibody targeting AKT in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n60203-2-Ig targets AKT in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, rat liver tissue,  mouse brain tissue,  HEK-293 cells,  NIH\/3T3 cells,  RAW 264.7 cells,  ROS1728 cells,  LNCaP cells,  Hela cells,  Jurkat cells,  HSC-T6 cells,  PC-12 cells\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human breast cancer tissue,  human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine\/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with\/without phospho- modification.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, rabbit, chicken, bovine, hamster, sheep, goat, duck\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag16695 Product name: Recombinant human AKT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-224 aa of BC000479 Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC Predict reactive species\nFull Name: v-akt murine thymoma viral oncogene homolog 1\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 56-62 kDa\nGenBank Accession Number: BC000479\nGene Symbol: AKT1\nGene ID (NCBI): 207\nRRID: AB_10912803\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P31749\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887967555753,"sku":"60203-2-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-60203-2-ig","provider":"Iright","version":"1.0","type":"link"}