{"product_id":"proteintech-60293-1-ig","title":"Proteintech, 60293-1-Ig, OPTN\/Optineurin Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe OPTN\/Optineurin (60293-1-Ig) by Proteintech is a Monoclonal antibody targeting OPTN\/Optineurin in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, pig samples\n60293-1-Ig targets OPTN\/Optineurin in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HeLa cells,  HEK-293 cells,  pig skeletal muscle tissue\nPositive IHC detected in: human pancreas cancer tissue,  human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nOPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag1272 Product name: Recombinant human OPTN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 233-577 aa of BC013876 Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII Predict reactive species\nFull Name: optineurin\nCalculated Molecular Weight: 66 kDa\nObserved Molecular Weight: 70-74 kDa\nGenBank Accession Number: BC013876\nGene Symbol: OPTN\nGene ID (NCBI): 10133\nRRID: AB_2881408\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q96CV9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888047018153,"sku":"60293-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-60293-1-ig","provider":"Iright","version":"1.0","type":"link"}