{"product_id":"proteintech-60666-1-ig","title":"Proteintech, 60666-1-Ig, Phospho-JNK (Thr183\/Tyr185) Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-JNK (Thr183\/Tyr185) (60666-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-JNK (Thr183\/Tyr185) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n60666-1-Ig targets Phospho-JNK (Thr183\/Tyr185) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  NIH\/3T3 cells,  HSC-T6 cells,  UV treated HEK-293 cells,  UV treated NIH\/3T3 cells,  UV treated HSC-T6 cells,  Anisomycin treated HeLa cells\nPositive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK\/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, chicken\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 8\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 42 kDa, 50 kDa\nGenBank Accession Number: NM_138982\nGene Symbol: JNK\nGene ID (NCBI): 5599\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P45983\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887973126313,"sku":"60666-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-60666-1-ig","provider":"Iright","version":"1.0","type":"link"}