{"product_id":"proteintech-65181-1-ig","title":"Proteintech, 65181-1-Ig, Anti-Human CD146\/MCAM (P1H12)","description":"Size: 100ug \/ 500ug\nThe CD146\/MCAM (65181-1-Ig) by Proteintech is a Monoclonal antibody targeting CD146\/MCAM in IF\/ICC, FC applications with reactivity to human, mouse, rabbit, canine samples\n65181-1-Ig targets CD146\/MCAM in WB, IF\/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IF\/ICC detected in: HUVEC cells\nPositive FC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rabbit, canine\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1, kappa\nClass: Monoclonal\nType: Antibody\nImmunogen: cultured human umbilical cells Predict reactive species\nFull Name: melanoma cell adhesion molecule\nCalculated Molecular Weight: 646 aa, 72 kDa\nGenBank Accession Number: BC056418\nGene Symbol: CD146\nGene ID (NCBI): 4162\nRRID: AB_2881483\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Purified by protein-A affinity chromatography\nUNIPROT ID: P43121\nStorage Buffer: PBS with 0.09% sodium azide, pH 7.3.\nStorage Conditions: Store at 2-8°C. Stable for one year after shipment.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888013824169,"sku":"65181-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-65181-1-ig","provider":"Iright","version":"1.0","type":"link"}