{"product_id":"proteintech-66180-1-ig","title":"Proteintech, 66180-1-Ig, CD71 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe CD71 (66180-1-Ig) by Proteintech is a Monoclonal antibody targeting CD71 in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications with reactivity to human samples\n66180-1-Ig targets CD71 in WB, IHC, IF\/ICC, IF-P, IP, ELISA, Blocking assay applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U-251 cells, A549 cells,  HeLa cells,  Raji cells,  TF-1 cells,  U2OS cells,  A431 cells,  HepG2 cells,  K-562 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human placenta tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human breast cancer tissue, MCF-7 cells\nPositive IF\/ICC detected in: MCF-7 cells, K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag21612 Product name: Recombinant human CD71 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 513-757 aa of BC001188 Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID Predict reactive species\nFull Name: transferrin receptor (p90, CD71)\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 90 kDa\nGenBank Accession Number: BC001188\nGene Symbol: CD71\nGene ID (NCBI): 7037\nENSEMBL Gene ID: ENSG00000072274\nRRID: AB_2881575\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P02786\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887981940905,"sku":"66180-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-66180-1-ig","provider":"Iright","version":"1.0","type":"link"}