{"product_id":"proteintech-66200-1-ig","title":"Proteintech, 66200-1-Ig, Acetyl-Tubulin (Lys40) Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Acetyl-Tubulin (Lys40) (66200-1-Ig) by Proteintech is a Monoclonal antibody targeting Acetyl-Tubulin (Lys40) in WB, IHC, IF\/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, canine samples\n66200-1-Ig targets Acetyl-Tubulin (Lys40) in WB, IHC, IF\/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat testis tissue, NCCIT cells,  Neuro-2a cells,  L02 cells,  pig cerebellum tissue,  rat brain tissue,  mouse ovary tissue,  HEK-293 cells,  mouse testis\nPositive IHC detected in: mouse ovary tissue, human gliomas tissue,  rat brain tissue,  mouse brain tissue,  human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: islet, mouse brain tissue\nPositive IF\/ICC detected in: MDCK cells, hTERT-RPE1 cells\nPositive ELISA detected in: Recombinant protein\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)-P: IF-P : 1:10-1:100\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nWhat is the molecular weight of acetyl-α-tubulin?The molecular weight of acetylated tubulin is 52 kD.Where does the acetylation of Lys 40 occur?This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).How can acetylation be reversed?Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).What is the function of acetylation?Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).Is acetylation of α-tubulin strictly associated with stable microtubules?Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).Is ac-tubulin found only in cilia?Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).What are the cellular effects of tubulin acetylation?Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, canine\nCited Reactivity: human, mouse, rat, pig, rabbit, canine, zebrafish, bovine, drosophila\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tubulin, alpha 1a\nCalculated Molecular Weight: 52 kDa\nObserved Molecular Weight: 50-55 kDa\nGenBank Accession Number: NM_006009\nGene Symbol: Alpha Tubulin\nGene ID (NCBI): 7846\nRRID: AB_2722562\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q71U36\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887972733097,"sku":"66200-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-66200-1-ig","provider":"Iright","version":"1.0","type":"link"}